Processing and annotation

After import data is provided into the dashboard, import of the sample into the project table is started by pressing the Add sample button. This may be an resource intensive task depending on the size of the input data and may take a minute to execute. During import, MetaPepView performs the following processing tasks:

• Wrangling the input data into a consistent format that is workable for MetaPepView to visualize.
• Grouping and quantifying peptides from scan counts and signal intensities. Grouping is done per sample.
• Checking compatibility of the new sample import sources with those present in the project table.
• Annotating DB search and de novo metaproteomics data with taxonomy information and functional information from the supplied datasets.
• Update the project table with the new sample data.

A high level overview of the importer and annotation pipeline executed during sample import is provided here.