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Processing and annotation for the compositions and functions dataset

After import data is provided for a new sample in the compositions and functions dataset, import of the sample into the dataset is started by pressing the Add sample button. This may be an resource intensive task depending on the size of the input data and may take a minute to execute. During import, meta-PepView performs the following processing tasks:

  • Wrangling the input data into a consistent format that is workable for meta-PepView to visualize.
  • Grouping and quantifying peptides from scan counts and signal intensities. Grouping is done per sample.
  • Checking compatibility of the new sample import sources with those present in the project table.
  • Annotating DB search and de novo metaproteomics data with taxonomy information and functional information from the supplied datasets.
  • Update the project table with the new sample data.

A high level overview of the importer and annotation pipeline executed during sample import is provided here.